![]() Membrane-compatible – colored bands transfer to membranes for Western blottingĭye-stained proteins in 62.5mM Tris-H 3PO 4 (pH 7.5 at 25☌), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN 3 and 33% glycerol. OctoBy Excedr Protein ladders are used in experiments to estimate the size of proteins of interest and are resolved using gel electrophoresis. Quality tested – each lot evaluated by SDS-PAGE and Western blottingīright reference bands – orange at 70 and 25kDa, and green at 10kDa The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce. Depending on the primary antibody, is sometimes possible to detect also the protein ladder, this does not means that your western blot is wrong or that the bands that you see are unspecific. Sharp bands – color-coded bands of similar intesity for easy visualization Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine blue-, orange-, and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. Ready-to-use – supplied in a loading buffer for direct loading on gels no need to boil Size range – nine proteins spanning 10 to 250kDa PageRuler Plus Prestained Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required before loading onto a gel. A blue chromophore is bound to all proteins, except proteins of two reference bands of 70kDa and 25kDa that are colored with an orange dye and one green reference band of 10kDa. ![]() This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Additional data should be considered for a more accurate adjustment.Thermo Scientific™ PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Consisting of recombinant proteins fused to an IgG binding site, these versatile molecular weight ladders bind the primary or secondary antibody used for the detection of the target protein. Under suggested conditions, Unstained Protein Ladder, Broad Range resolves 12 major bands in 4-12% Bis- Tris Gel (MES buffer) and after Western blotting to the nitrocellulose membrane. Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |